Coding
YFP

Part:BBa_K2984075

Designed by: Dimitri Schumacher   Group: iGEM19_Humboldt_Berlin   (2019-10-19)


YFP - Yellow fluorescent protein for detection

Yellow fluorescent protein mVenus for in vivo detection of proteins in C. reinhardtii or its surroundings. The YFP mVenus has an excitation peak at 515 nm and an emission peak at 528 nm (Kremers et al. 2006)

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 202
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 202
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 202
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 202
    Illegal NgoMIV site found at 679
  • 1000
    COMPATIBLE WITH RFC[1000]


MoClo Overhangs

This part with B3-B4 MoClo overhangs can be found here.

This part with B4-B4 MoClo overhangs can be found here.

This part with B5-B5 MoClo overhangs can be found here.


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Categories
Parameters
None